Influence of Secnedazol on the Pharmacokinetics of Diminazene Aceturate in Dogs Infected with Trypanosoma Brucei: A Crucial Study for Enhancing Treatment Efficacy
Abstract
This study evaluated the pharmacologic effect of Diminazene aceturate and Secnidazol combination in dogs infected with trypanosome brucei and effect of Secnidazol in pharmacokenitics disposition of DA in infected dogs. Trypanosome brucei stock used was obtained from natural breed of dogs and infected in albino rats in which the Trypanasome is stored and was kept at the department of Veterinary Parasitology and Entomology University of Nigeria Nsukka.18 dogs was used in the Experiment and the dogs were randomly selected from both sexes between the age of 5-10 months into five groups, four of the groups were infected and only one was uninfected.The dogs were experimentally infected by intraperitoneal injection of 5x10 trypanosome in 1 ml of phosphate buffer saline. Effect of combination of SEC and DA was studied on Parasitemia, heamatology T. brucei infected dogs.Data on parasitemia and Heamatology were analysed with one way analysis of variance. Variant means were separated Post-hoc using the Least Significant Difference methods. The level of significance was accepted at p ≤ 0.05. Pharmacokinetic data for DA alone and DA plus SEC combination were analysed using Independent sample T.test significant value was accepted at p<0.05. The effect on parasitemia in SEC and DA treated group was lower on day 7 than group treated with DA alone and there was no parasitemia in all the groups on day 21. on heamatology /there was significant difference between the group on PCV,RBC and Hb on day7 but no difference on day 14. A mean pick serum concentration of 1.29±0.12ug/ml was achieved at 0.5h after drug administration in dog pretreated with SEC. However as 0.25h in DA treated alone serum concentration observed was 1.23±0.04 ug/ml. The T max for DA plus SEC group was 0.5h against 0.25h for DA group. AUC of dog pretreated with SEC significantly(p<0.05) lower than the AUC of dogs treated with DA alone, there was no significant difference in CPO, Ka, Ke, T1/2Ke, Vd Cl and Cmax, there was no significant difference between the DA concentration.But DA values of CPO, Ka and T1/2Ke were higher than SEC plus DA whereas T1/2 Ka, Vd,Cl Tmax and Cmax was higher in sec pretreated. It can be concluded from the pharmacokinetics study that SEC improved the efficacy of DA by altering the pharmacokinetic constants. There was an improvement in the absorption and elimination of DA in the group that received heterotherapy treatment, hence heterotherapy treatment of SEC and DA is more efficient in the treatment of T.brucei in infected dogs compared to when a monotherapy of DA is used in the treatment of the disease.
Keywords
Pharmacologic effect. Diminazene aceturate & Secnidazol combination. Dog Trypanosomosis
References
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