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Phytochemical, Vitamin Composition and Bacterial Contamination of Pleurotus Ostreatus and Pleurotus Tuber-Regium

Owabie, P. I, Wala Chimela

Abstract

Studies on the phytochemical composition and bacterial contamination of Pleurotus ostreatus and Pleurotus tuber-regium grown in Port Harcourt were carried out in the Department of Plant Science and Biotechnology Rivers State University. Fresh and Dry P.ostreatus and P.tuber- regium were grown and harvested from Dilomat farms Rivers State University Port Harcourt. The samples were collected and analyzed in Food Science and Biotechnology Laboratory. Mycological studies were carried out in Department of Plant Science Biotechnology Laboratory using standard methods. The result for phytochemical composition revealed phytate, oxalate, saponin, tannin, carotenoid, polyphenol, flavonoid and lignin. Fresh P. ostreatus and Fresh P. tuber-regium had similar phytate value (0.01±0.00), Fresh P. ostreatus recorded high value for oxalate (0.04±0.00), tannin (3.12±0.00), carotenoid (30.56±0.05), while Fresh P. tuber-regium had high value for saponin (0.02±0.00), polyphenol (7.53±0.05), flavonoid (3.53±0.05) and lignin (5.83±0.5). Vitamin composition results revealed the presence of vitamin A and thiamin. Fresh P. ostreatus had higher vitamin A (3.00±0.00) and thiamin (0.16±0.00) while Dry P. ostreatus recorded (2.50±0.00) for vitamin A and (0.18±0.01) for thiamin respectively. Bacterial contamination from Fresh and Dry P. ostreatus identified include E. coli and Bacillus sp while Fresh and Dry P. tuber-regium revealed pseudomonas and bacillus sp respectively. P. ostreatus and P. tuber-regium are rich in phytochemical and vitamin composition. However, they are prone to contamination, and as such, care must be taken during growing and handling of harvested mushrooms to prevent contamination. More so, mushrooms with signs of contaminations should not be consumed to avoid bacterial infection. Key word: Phytochemical composition, Bacterial contamination, Pleurotus ostreatus, Pleurotus tuber-regium.

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