IIARD INTERNATIONAL JOURNAL OF GEOGRAPHY AND ENVIRONMENTAL MANAGEMENT (IJGEM )

E-ISSN 2504-8821
P-ISSN 2695-1878
VOL. 11 NO. 5 2025
DOI: 10.56201/ijgem.vol.11.no5.2025.pg58.69


Histology Based Whole Effluent Chronic Toxicity Testing of Noodles Processing Company Waste, using the Liver of Clarias gariepinus, as a Biomarker

Theodore A Allison, and Ebibouloukami G Waritimi


Abstract


This study investigated the chronic toxicity of effluents from a noodle food industry, focusing on the environment water quality and its histological impact on the liver of Clarias gariepinus. The following known aquatic contaminants from food industries were selected as the effluent target chemical (TC) of concern: cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and polyaromatic hydrocarbons (PAH). Effluent concentrations of 6.25%, 12.5%, 25%, 50%, and 100% were tested, with a control group maintained in clean water. Histopathological analysis of the liver was conducted to assess structural alterations linked to effluent toxicity. Effluent analysis result revealed Results revealed that Cu levels (0.037 mg/L) exceeded permissible regulatiro thresholds, highlighting significant contaminantion concerns. Histological analysis revealed graded liver damage, highlighting the significant impact of the studied industrial effluents on aquatic life.


keywords:

Histology; Liver, Effluent; Toxicology, Biomarker; Fish and Water Quality.


References:


Ackermann, C. (2008). A quantitative and qualitative histological assessment of selected
organs of Oreochromis mossambicus after acute exposure to cadmium, chromium and
nickel. MSc dissertation. University of Johannesburg: Johannesburg.
Ahmed, T., Noman, M., Ijaz, M., Ali, S., Rizwan, M., Ijaz, S., Hameed, A., Ahmad, S., Wang,
Y., Sun, G., & Li, B. (2021). Current trends and future prospective in nanoremediation
of heavy metals contaminated soils: A way forward towards sustainable agriculture.
Ecotoxicology and Environmental Safety, Volume 227,112888.
Ajima, M. N. O., Ogo, A. O., Audu, B. S., & Ugwoegbu, K. C. (2015) Chronic diclofenac
(DCF) exposure alters both enzymatic and haematological profile of African catfish,
Clarias gariepinus. Drug Chem. Toxicol. 38(4), 383-390.
Allison, T. A., & Paul, C. W. (2014). Histological based biomonitoring: a baseline
ecotoxicological
evaluation
of
New-Calabar
River
using
Chrysichthys
nigrodigitatus. Int J Environ Poll Res, 2(3), 17-41.
Allison, T. A., & Ogoun, T. R., (2024). Histological Based Environmental Monitoring of the
Lower Nun River: Using the Histo-morphometry of Kidney, Gills, Liver, and Testes of
Coptodon zillii. Journal of Advances in Medical and Pharmaceutical Sciences Volume
26, Issue 10, Page 20-39, 2024; Article no.JAMPS.117884 ISSN: 2394-1111
Allison, T. A., Isirima, J. C., Osunwoke, E. A., & Bob-manuel, I. F. (2023). Allison's
Toxicology Histopathology: Volume 1- Lesion Pathomechanism and Diagnosis. Igiri
press and libraries (IPL). ISBN 9789781022951
ATSDR (Agency for Toxic Substances and Disease Registry), (2012a). Public Health
Statement on Chromium. Department of Health and Human Services, Public Health
Service Agency for Toxic Substances and Disease Registry
Bashir, I., F. A., Lone, F. A., & Bhat, R. A. (2020). Concerns and Threats of Contamination on
Aquatic Ecosystems. In book: Bioremediation and Biotechnology, Sustainable
Approaches to Pollution Degradation (pp.1-26). DOI:10.1007/978-3-030-35691-0_1
Birge, W. J., Blac, J. A., Short, T. M., & Wester- man, A. G. (1989). A Comparative Ecological
and Toxicological Inves- tigation of a Secondary Wastewater Treatment Plant Efflux-
ent and Its Receiving Stream. Environmental Toxicology and Chemistry, Vol. 8, No. 5,
1989, pp. 437- 450.
Borgmann, U., Cheam, V., Norwood, W. P., & Lechner, J. (1998). Toxicity and bioaccumulation
of thallium in Hyalella azteca, with comparison to other metals and prediction of
environmental impact. Environmental Pollution, Volume 99, Issue 1, Pages 105-114.
Cao, P., Lu, C., & Yu, Z. (2017). Agricultural nitrogen fertilizer uses in the continental U.S.
during 1850-2015: a set of gridded time-series data [dataset]. PANGAEA,
https://doi.org/10.1594/PANGAEA.883585,
CEPA (California Environmental Protection Agency), (2004). Overview of Freshwater and
Marine Toxicity Tests: A Technical Tool for Ecological Risk Assessment.
Ecotoxicology Unit Reproductive and Cancer Hazard Assessment Section Office of
Environmental Health Hazard Assessment California Environmental Protection
Agency.
Chan, F. K. M., Moriwaki, K., & De Rosa, M. J. (2013). Detection of necrosis by release of
lactate dehydrogenase activity. Immune Homeost. Methods Protoc. 979, 65–70.
Chang, X., Kobayashi, T., Senthilkumaran, B., Kobayashi-Kajura, H., Sudhakumari, C. C., &
Nagahama, Y. (2005). Two types of aromatase with different encoding genes, tissue
distribution and developmental expression in Nile Tilapia (Oreochromis mossambicus).
Gen. Comp. Endocrinol., 141: 101-115.
IIARD International Journal of Geography & Environmental Management
Vol. 11 No. 5 2025 E-ISSN 2504-8821 P-ISSN 2695-1878 www.iiardjournals.org
IIARD – International Institute of Academic Research and Development
Page 68
Chindah, A. C., Braids, S. A., & Obunwo, C. (2011). Water Quality of Streams Receiving
Municipal Waste Water in Port Harcourt, Niger Delta, Nigeria. In book: Waste Water
- Evaluation and Management. DOI:10.5772/16186
Cullen J. M. (2005). Mechanistic classification of liver injury. Toxicol Pathol ;33:6–8.
Doonan, F., & Cotter T. G. (2008). Morphological assessment of apoptosis.
Methods ;44(3):200-4.
DWAF (Department of Water Affairs and Forestry), (1996). South African Water Quality
Guidelines Volume 7. Aquaticecosystem Pretoria: DWAF Ecotoxicology and
Environmental Safety. 1(2): 151
Ebol, E. L., Donoso, C. H., Saura, R. B. D., Ferol, R. J. C., Mozar, J. R. D., Bermon, A. N.,
Manongas, J., Libot, J. C. H., Cara, J., Matabilas, C. J., Jumawan, J. C., &
Capangpangan, R. Y. (2020). Heavy Metals Accumulation in Surface Waters, Bottom
Sediments and Aquatic Organisms in Lake Mainit, Philippines. Inter. Letters Nat. Sci.
79: 40-49 Edokpayi, JN; Odiyo, JO; Popoola, OE; Msa
Eagleson K. W. et al. 1990. Comparison of measured instream biological responses with
responses predicted using the Ceriodaphnia dubia chronic toxicity test. Environ.
Toxicol. Chem. 9: 1019-1028.
EEA (European Environmental Agency) (2018). Air quality in Europe - 2018 Report. EN PDF:
TH-AL-18-013-EN-N - ISBN: 978-92-9213-990-2
Drury and Wallington (1980).Drury, R.A. and Wallington, E.A., (1980). Carleton’ s Histology
Technique. 5th Edition, Oxford University Press, Newyork.
Guengerich, F. P. (2003). Cytochrome P450 oxidations in the generation of reactive
electrophiles: epoxidation and related reactions. Arch Biochem Biophys: 409(1):59-71.
ISO (International Organization for Standardization) (1994). Methods of determining the long-
term toxicity of substances to freshwater fish. ISO-10390: Geneve, Switzerland
Karin, M. & Dhar, D. (2016). Liver carcinogenesis: from naughty chemicals to soothing fat
and the surprising role of NRF2, Carcinogenesis, Volume 37, Issue 6: Pages 541–546.
Lehman-McKeeman, L. D., (2013). Systems and computational biology as foundations for
toxicology research. Toxicologist 132: 821
Liao, M., Zhang, H., Yu, S., Chen, C., Huang, C., Xu, J., & Huang, P. (2010). Effects of
cadmium and mercury alone and in combination on the soil microbial community
structural diversity. J. Xu, P. Huang (Eds.), Molecular Environmental Soil Science at
the Interfaces in the Earth's Critical Zone, Springer, Berlin, Heidelberg, pp. 337-341
Marchand, M. J, van Dyk, J..C, Pieterse, G. M., Barnhoorn, E. J., & Bornman, M. S. . (2009).
Histopathological alterations in the liver of the sharptooth catfish Clarias gariepinus
from polluted aquatic systems in South Africa. Environmental Toxicology 24: 133–147.
McKim, J. M. (1977)..Evaluation of Tests with Early Life Stages of Fish for Predicting Long-
Term Toxicity. Journal of the Fisheries Research Board of Canada, 34, 1148-1154.
McIntyre, J. K, Davis, K. W., Hinman, C., Macneale, K. H., Anulacion, N. F. Scholz, N. L., &
Stark, J. D. (2015). Soil bioretention protects juvenile salmon and their prey from the
toxic impacts of urban stormwater runoff. Chemosphere, Volume 132: Pages 213-219.
Mustafa, A.D., Juahir, H., Yunus, K., Amran, M. A., Hasnam, C. N. C., Azaman, F., Abidin, I.
Z., Azmee, S. H. & Sulaiman, N.H. (2015). Oil spill related heavy metal: a review.
Malaysian. Journal of Analytical Sciences. 19(6): 1348 1360.
Nikinmaa, M. (2014). An introduction to aquatic toxicology. Elsevier Inc. Academic Press.
Nwankwoala H. O. & Angaya Y. B., (2017). An Evaluation of Heavy Metals Concentration in
the Choba Section of the New Calabar River, Eastern Niger Delta. Scientific Review,
Academic Research Publishing Group, vol. 3(6): pages 54-61.
IIARD International Journal of Geography & Environmental Management
Vol. 11 No. 5 2025 E-ISSN 2504-8821 P-ISSN 2695-1878 www.iiardjournals.org
IIARD – International Institute of Academic Research and Development
Page 69
OECD (The Organization for Economic Cooperation and Development), (2013). OECD
Guidelines for the Te


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