RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY (RJPST )
E-ISSN 2579-0536
P-ISSN 2695-2696
VOL. 8 NO. 5 2025
DOI: 10.56201/rjpst.vol.8.no5.2025.pg94.103
Theodore Athanasius Allison and Ebibouloukami Gilbert Waritimi
Industrial effluents discharged into aquatic environments pose as a significant ecological and biological threats, particularly to aquatic species like Clarias gariepinus. This study investigated the chronic toxicity of effluents from the noodles food company on the histology of the female gonads of Clarias gariepinus. The following known aquatic contaminants from food industries were selected as the effluent target chemical (TC) of concern: cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and polyaromatic hydrocarbons (PAH). Effluent samples were analyzed for TC constituents, and their effects on fish were assessed under varying concentrations (6.25%, 12.5%, 25%, 50%, and 100%). Results revealed that Cd levels (0.037 mg/L) exceeded permissible regulatory thresholds, highlighting significant contamination concerns. Histological analysis of the female gonads showed significant histological damage, including oocyte degeneration, atretic, follicles, and disrupted ovarian architecture. This findings highlight the detrimental impact of untreated or inadequately treated effluent on aquatic ecosystems and underscores the need for stricter effluent discharge regulations
Histology; Female Gonad, Effluent; Toxicology, Biomarker; Fish and Water Quality.
Adebayo, O. T., & Fawole, O. O. (2014). Histological alterations in the gonads of Clarias
gariepinus exposed to industrial effluents. African Journal of Aquatic Science, 39(2), 149–
Adeoye, G. O., Alimba, C. G., & Oyeleke, C. (2015). The genotoxicity and systemic toxicity of a
pharmaceutical effluent in Wistar rats involve in oxidative stress induction. Toxicol Rep.;
1265-72
Agunbiade, F., & Moodley, B. (2016). Occurrence and distribution pattern of acidic
pharmaceuticals in surface water, wastewater, and sediments of the Nsun duzi River,
Kwazulu-Natal, South Africa. Environmental Toxicology and Chemistry; 35, 36-46.
Alimba, C. G., Ajayi, E. O., Hassan, T., Sowunmmi, A. A., & Bakare, A. A. (2015).
Cytogenotoxocity of abattoir effluent in Clarias gariepinus (Burchell, 18222) using
micronucleus test. Chinese J. Biol.
Alimba, C. G., Ajiboye, R. D., & Fagbenro O. S. (2017). Dietary ascorbic acid reduced
micronucleus and nuclear abnormalities in Clarias gariepinus (Burchell, 1822) exposed to
hospital effluent. fish physiological biochemistry; 43: 1325-1335.
Annune, P. A., Ebele, S. O., & Oladimeji, A. A. (1994). Acute toxicity of cadmium to juveniles of
Clarias gariepinus (Teugels) and Oreochromis niloticus (Trewavas). Journal of
Environmental Science and Health Part A, 29(7), 1357-1365.
Drury, R. A., & Wallington, E. A., (1980). Carleton’s Histology Technique. 5th Edition, Oxford
University Press, New York.
Emmanuel, S. E., & Aluko, P. O. (2021). Histological study of the gonads of Clarias gariepinus
exposed to agricultural pesticides. Environmental Science and Pollution Research, 28(12),
14234-14244.
Jaishankar, M., Tseton, T., & Anbalagan, N. (2014). Toxicity Mechanism and Health Effects of
some Heavy Metals. Interdisciplinary Toxicology, 7(2), 60-70.
Jobling, S. & Tyler, C. R. (2003). Endocrine disruption in wild freshwater fish. Pure Appl. Chem.,
75:2219-2234.
Luo, Y., Shan, D., Zhong, H., Zhou, Y., Chen, W., Cao, J., ... & Huang, Y. (2015). Subchronic
effects of cadmium on the gonads, expressions of steroid hormones and sex-related genes
in tilapia Oreochromis niloticus. Ecotoxicology, 24(10), 2213-2223.
OECD (Organisation for Economic Co-operation and Development 210), (2013). OECD
Guidelines for the Testing of Chemicals: Test (No. 210), Fish, Early-life Stage Toxicity.
Organisation for Economic Co-operation and Development, Paris, France.
Olufayo, M. O., & Alade, A. A. (2012). Histopathological alterations in the gonads of Clarias
gariepinus exposed to Cadmium. African Journal of Aquatic Science, 37(2), 145-152.
Olojo, E. A. A., Olurin, K. B., Mbaka, G., & Oluwemimo, A. D. (2005). Histopathology of the gill
and liver tissues of the African catfish Clarias gariepinus exposed to lead. African Journal
of Biotechnology, 4(1), 117-122.
Rand G. M., & Petrocelli S. R. (Eds), (1985). Fundamentals of Aquatic Toxicology. Methods and
Applications. Hemisphere, New York. pp. 374-415.
Sastry B. V. R. (1991). Placental toxicology: tobacco smoke, abused drugs, multiple chemical
interactions, and placental function. Reproduction, Fertility, and Development; 3(4):355–
[PubMed]
USEPA (United State Environmental Protection Agency), (2002). Short-term Methods for
Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and
Estuarine Organisms. Third Edition. EPA-821-R-02-014